. 2011 Nov 11;72(3):206–223. doi: 10.1159/000331585

Table 5.

Associations between SNPs in CD46, SLAM and CD209 genes and secreted cytokine immune responses to measles vaccine in the combined, Caucasian and African-American study subjects

Secreted cytokine/Gene	SNPID	Location/function	Genotyp e^a	n	Median, pg/ml (IQR)^b	p value^c
IL-2
Combined cohort of subjects (n = 745)
None

Caucasian subgroup fn = 598)
SLAM	rs2295614	flanking 5′ UTR	TT	514	42.4 (23.3; 66.8)	0.0303
			TA	76	34.4 (19.0; 65.9)
			AA	2	31.5 (17.7; 45.3)

African-American subgroup (n = 89)
CD46	rs2724382	intron	GG	33	28.9 (16.8; 67.6)	0.0117
			GC	44	31.1 (15.3; 46.4)
			CC	11	15.2 (8.4; 36.6)

CD209	rs8112310	flanking 3′ UTR	TT	24	25.3 (11.8; 48.6)	0.0170
			TA	62	30.1 (15.6; 52.8)
			AA	0	–

CD46	rs2796268	flanking 5′ UTR	AA	28	32.8 (15.3; 82.1)	0.0282
			AG	45	30.6 (17.2; 43.4)
			GG	15	15.4 (11.3; 36.6)

CD209	rs4804806	flanking 5′ UTR	GG	33	36.6 (18.5; 59.2)	0.0297
			GA	37	22.7 (12.3; 50.6)
			AA	18	29.0 (15.2; 35.1)

CD46	rs2724385	intron	AA	40	27.2 (13.9; 51.9)	0.0331
			AT	44	27.9 (15.9; 46.4)
			TT	4	44.1 (33.8; 75.2)

SLAM	rs3766362	intron	AA	30	15.7 (11.3; 46.6)	0.0381
			AT	38	29.0 (17.0; 52.8)
			TT	20	36.7 (22.8; 47.7)

SLAM	rs6704124	intron	GG	52	25.6 (12.2; 43.2)	0.0412
			GA	30	29.8 (17.0; 52.8)
			AA	6	43.6 (18.5; 65.6)

IL-6
Combined cohort of subjects (n = 745)
CD46	rs2796267	flanking 5′ UTR	AA	270	359.3 (257.6; 479.8)	0.0006
			AG	343	353.8 (236.4; 458.3)
			GG	123	341.2 (245.2; 446.9)

CD46	rsllll8516	intron	GG	640	361.2 (253.7; 469.7)	0.0133
			GA	92	305.4 (206.7; 401.0)
			AA	1	348.1 (348.1; 348.1)

CD46	rs2724384^*	intron	AA	447	348.4 (245.8; 458.0)	0.0275
			AG	260	365.5 (253.5; 470.1)
			GG	30	381.6 (269.5; 498.4)

CD46	rs41316827	intron	CC	710	354.6 (248.4; 461.4)	0.0493
			CA	26	348.5 (221.0; 469.5)
			AA	1	332.2 (332.2; 332.2)

Caucasian subgroup (n = 598)
CD46	rs2796267	flanking 5′ UTR	AA	203	356.2 (254.6; 469.5)	0.0042
			AG	281	351.3 (240.2; 457.6)
			GG	107	351.5 (218.4; 454.9)

African-American subgroup (n = 89)
CD46	rsllll8516	intron	GG	75	348.9 (247.8; 462.4)	0.0012
			GA	13	295.5 (224.9; 303.5)
			AA	0	–

CD46	rs2796267	flanking 5′ UTR	AA	45	358.9 (287.6; 479.8)	0.0180
			AG	34	304.3 (230.9; 418.9)
			GG	9	286.1 (269.5; 295.5)

CD209	rs4804806	flanking 5′ UTR	GG	33	364.9 (269.8; 480.9)	0.0254
			GA	37	318.4 (230.7; 399.8)
			AA	18	321.9 (247.8; 436.0)

SLAM	rs2753260	intron	AA	51	340.2 (262.5; 477.6)	0.0313
			AC	32	314.3 (227.8; 382.1)
			CC	5	358.9 (238.7; 370.6)

IL-10
Combined cohort of subjects (n = 745)
None

Caucasian subgroup (n = 598)
CD209	rsl2611071	flanking 5′ UTR	CC	452	17.8 (11.9; 28.2)	0.0246
			CA	129	19.8 (12.4; 30.2)
			AA	13	21.4 (17.4; 29.6)

African-American subgroup (n = 89)
SLAM	rsl 1265452^*	intron	GG	31	12.1 (7.4; 22.2)	0.0008
			GA	31	13.3 (7.4; 28.6)
			AA	26	13.9 (7.9; 26.2)

SLAM	rs2089088	intron	AA	66	10.9 (7.4; 20.6)	0.0232
			AC	18	22.2 (12.8; 35.0)
			CC	4	16.4 (10.1; 31.2)

IFN-α
Combined cohort of subjects (n = 745)
CD46	rs2724384^*	intron	AA	445	506.4 (244.5; 935.1)	0.0068
			AG	259	618.9 (321.8; 1,197.4)
			GG	30	657.3 (344.5; 1,338)

CD46	rs41318019	flanking 3′ UTR	GG	645	567.2 (273.4; 1,047.4)	0.0330
			GC	87	509.9 (272.8; 846.6)
			CC	2	262.5 (1.5; 523.5)

CD46	rsllll8516	intron	GG	637	559.3 (275.2; 1,025.3)	0.0401
			GA	92	491.2 (244.3; 1,028.8)
			AA	1	210.2 (210.2; 210.2)

CD209	rs7250738	flanking 5′ UTR	AA	677	550.2 (264.4; 1,018.0)	0.0427
			AG	51	581.5 (393.3; 1,245.8)
			GG	6	876.2 (271.9; 1,678.0)

Caucasian subgroup (n = 598)
CD46	rs2724384^*	intron	AA	338	545.8 (242.1; 955.7)	0.0016
			AG	223	661.9 (332.6; 1,220.6)
			GG	27	703.0 (243.5; 1,338)

CD46	rsllll8516	intron	GG	521	593.9 (290.4; 1,093.5)	0.0072
			GA	62	679.1 (281.6; 1,012)
			AA	1	210.2 (210.2; 210.2)

CD46	rs2724382	intron	GG	201	459.1 (225.1; 941.9)	0.0084
			GC	272	638.6 (328.2; 1,091.2)
			CC	115	678.9 (312.6; 1,186.3)

CD46	rs2796268	flanking 5′ UTR	AA	196	462.4 (223.5; 963.9)	0.0133
			AG	269	639.6 (329.6; 1,101.5)
			GG	118	670.4 (295.4; 1,156.1)

CD46	rs2796267	flanking 5′ UTR	AA	201	480.7 (219.3; 919.6)	0.0148
			AG	279	639.6 (328.2; 1,093.5)
			GG	107	722.0 (282.5; 1,230.1)

CD46	rs7144	3′ UTR	AA	204	462.4 (223.5; 951.7)	0.0185
			AG	271	639.6 (328.2; 1,101.5)
			GG	113	661.9 (312.6; 1,156.1)

CD209	rsl2611071	flanking 5′ UTR	CC	448	591.9 (269.4; 1,051.6)	0.0288
			CA	127	581.5 (324.6; 1,093.5)
			AA	13	969.6 (839.4; 1,678.0)

African-American subgroup (n = 89)
SLAM	rsl7385878	flanking 3′ UTR	GG	47	293.6 (199.1; 535.4)	0.0163
			GA	35	450.6 (212.2; 805.2)
			AA	6	621.8 (515; 1,109.3)

SLAM	rs2295614	flanking 5′ UTR	TT	79	400.1 (212.2; 608.7)	0.0253
			TA	8	437.0 (241.5; 722.8)
			AA	1	1,571.2 (1,571.2; 1,571.2)

CD209	rs4804805	flanking 5′ UTR	AA	63	293.6 (195.1; 546.9)	0.0260
			AG	21	550.3 (430.5; 1,173.4)
			GG	4	506.6 (301.7; 636.0)

SLAM	rs5002947	flanking 3′ UTR	GG	35	293.6 (162.5; 608.7)	0.0396
			GA	45	400.1 (253.5; 580.9)
			AA	8	621.8 (488.8; 1,141.3)

CD46	rs41317049	intron	AA	80	417.5 (243.5; 659.4)	0.0480
			AC	7	321.8 (202.2; 498.1)
			CC	0	–

IFN-γ
Combined cohort of subjects (n = 745)
SLAM	rs2295614	flanking 5′ UTR	TT	628	68.7 (36.9; 120.5)	0.0128
			TA	101	58.9 (23.5; 118.2)
			AA	5	70.4 (13.1; 75.9)

CD46	rs2796267	flanking 5′ UTR	AA	270	63.6 (35.2; 123.3)	0.0174
			AG	343	66.7 (32.4; 112.2)
			GG	123	76.4 (40.7; 131.4)

Caucasian subgroup (n = 598)
None

African-American subgroup (n = 89)
CD209	rs4804806	flanking 5′ UTR	GG	33	62.2 (26.0; 109.2)	0.0007
			GA	37	53.4 (31.1; 75.4)
			AA	18	49.6 (14.4; 136)

CD46	rs2796268	flanking 5′ UTR	AA	28	46.6 (29.1; 133.1)	0.0012
			AG	45	62.8 (42.5; 97.8)
			GG	15	38.9 (6.4; 69.5)

CD46	rs2724382	intron	GG	33	48.2 (28.2; 115.4)	0.0072
			GC	44	62.0 (34.5; 97.2)
			CC	11	56.0 (6.2; 135.3)

SLAM	rs2295614	flanking 5′ UTR	TT	79	57.3 (25.5; 105.2)	0.0158
			TA	8	61.4 (47.8; 101.9)
			AA	1	70.4 (70.4; 70.4)

CD46	rs2796267	flanking 5′ UTR	AA	45	60.1 (30.1; 115.4)	0.0204
			AG	34	61.5 (26.1; 96.7)
			GG	9	50.5 (24.5; 59.0)

CD46	rs2724385	intron	AA	40	56.2 (17.6; 82.3)	0.0358
			AT	44	61.5 (39.4; 118.8)
			TT	4	60.6 (13.6; 167.0)

CD46	rs!7006738	intron	GG	63	57.3 (26.0; 97.8)	0.0473
			GA	23	61.3 (28.2; 139.3)
			AA	2	32.5 (3.5; 61.4)

IFNλ-1
Combined cohort of subjects (n = 745)
SLAM	rs2295614	flanking 5′ UTR	TT	629	33.1 (14.2; 72.4)	0.0027
			TA	101	39.9 (14.2; 87.5)
			AA	5	51.5 (−2.8; 126.8)

CD46	rs2724382	intron	GG	266	32.0 (12.6; 69.6)	0.0443
			GC	341	33.3 (14.8; 73.5)
			CC	131	40.3 (17.1; 86.7)

Caucasian subgroup (n = 598)
CD209	rsl2611071	flanking 5′ UTR	CC	452	37.5 (15.0; 73.1)	0.0027
			CA	127	44.5 (22.6; 85.7)
			AA	13	73.0 (36.2; 137.7)

SLAM	rs2295614	flanking 5′ UTR	TT	512	39.3 (17.0; 76.2)	0.0083
			TA	76	40.6 (16.8; 91.1)
			AA	2	188.8 (51.5; 326.1)

CD209	rs7248637	3′ UTR	GG	492	38.7 (16.5; 75.7)	0.0465
			GA	92	40.2 (21.3; 86.0)
			AA	6	71.2 (61.2; 163.9)

African-American subgroup (n = 89)
SLAM	rs!7385878	flanking 3′ UTR	GG	47	13.1 (3.0; 24.9)	0.0198
			GA	35	25.6 (−5.7; 48.8)
			AA	6	42.0 (21.5; 64.3)

TNF-α
Combined cohort of subjects (n = 745)
CD46	rs2724384^*	intron	AA	444	13.0 (8.5; 18.5)	0.0007
			AG	258	14.6 (10.1; 18.8)
			GG	30	16.8 (11.0; 22.9)

CD46	rs7144	3′ UTR	AA	264	12.8 (8.4; 17.6)	0.0020
			AG	339	14.0 (9.3; 18.8)
			GG	129	15.2 (10.7; 20.9)

CD46	rs2724385	intron	AA	192	14.0 (10.1; 20.3)	0.0030
			AT	357	14.1 (9.1; 18.8)
			TT	182	12.9 (8.8; 16.9)

CD46	rs2796268	flanking 5′ UTR	AA	251	12.9 (8.3; 18.3)	0.0048
			AG	337	13.9 (9.4; 18.8)
			GG	138	14.9 (10.5; 20.4)

CD46	rs2724382	intron	GG	263	12.9 (8.4; 17.9)	0.0143
			GC	340	14.0 (9.3; 18.8)
			CC	129	15.0 (10.6; 20.4)

Caucasian subgroup (n = 598)
CD46	rs2724384^*	intron	AA	337	13.1 (8.9; 18.2)	2.3104E⁻⁰⁵
			AG	223	15.2 (10.3; 18.9)
			GG	27	17.6 (11.0; 22.9)

CD46	rs7144	3′ UTR	AA	204	13.1 (8.6; 17.3)	0.0003
			AG	271	14.2 (9.7; 18.9)
			GG	112	15.3 (10.8; 20.7)

CD46	rs2796268	flanking 5′ UTR	AA	196	12.9 (8.4; 17.4)	0.0005
			AG	269	14.2 (10.0; 18.8)
			GG	117	15.3 (10.7; 20.4)

CD46	rs2724382	intron	GG	201	13.0 (8.5; 17.1)	0.0013
			GC	272	14.6 (10.0; 18.9)
			CC	114	15.3 (10.7; 20.4)

CD46	rs2724385	intron	TT	145	15.0 (10.6; 20.9)	0.0013
			TA	283	14.7 (9.5; 18.8)
			AA	158	12.9 (8.9; 16.5)

CD46	rs2796267	flanking 5′ UTR	AA	200	12.8 (8.9; 17.8)	0.0135
			AG	279	14.6 (10.2; 18.8)
			GG	107	15.5 (9.8; 20.4)

African-American subgroup (n = 89)
SLAM	rsl0489635	intron	GG	77	11.8 (7.7; 18.1)	0.0115
			GA	11	7.4 (1.5; 13.4)
			AA	0	–

CD46	rsllll8516	intron	GG	75	11.6 (6.4; 17.4)	0.0135
			GA	13	10.3 (8.0; 16.5)
			AA	0	–

CD46	rsl7006738	intron	GG	63	10.3 (5.7; 17.0)	0.0215
			GA	23	13.9 (8.7; 18.8)
			AA	2	23.4 (10.1; 36.6)

CD209	rs2287886	flanking 5′ UTR	GG	41	12.3 (8.0; 17.4)	0.0236
			GA	40	11.7 (6.0; 17.9)
			AA	7	9.1 (7.4; 13.4)

A = Adeninę; C = cytosine; G = guanine; T = thymine; IQR = interquartile range.

A total of 55 SNPs were examined; only those found to be statistically significant (p ≤ 0.05) were included in the table. SNP rs8112310 (p = 1.1972E⁻⁰⁷) displayed violations of HWE.

Values are presented as homozygous major allele/heterozygous/homozygous minor allele.

Values are median levels (IQR) in pg/ml as measured by ELISA.

Test for trend p value from the analysis of covariance adjusting for age, gender, race and age of immunization. The p values are adjusted for age, gender, race (when necessary), age at the first and second MMR and cohort status (cohort 1 vs. cohort 2) using analysis of covariance methodology.

Previously reported SNP associated with measles antibodies [24],