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. Author manuscript; available in PMC: 2011 Dec 19.
Published in final edited form as: Biochemistry. 2011 Sep 7;50(39):8374–8382. doi: 10.1021/bi2009079

Figure 2.

Figure 2

Colocalization of RNase A with endocytic markers. Confocal microscopy of HeLa cells incubated with fluorogenic RNase A (27) (10 μM) and (i) Alexa Flour 594–transferrin (1 μM), (ii) Alexa Flour 594–CTB (1 μg/mL), (iii) 10 μM TAMRA–R9 (10 μM), or (iv) TAMRA–TAT (10 μM) for 1 h at 37 °C, and washed. Incubations with CPPs followed a 15-min pretreatment with RNase A. Scale bars: 10 μm.