Figure 3.

Induction of serine protease inhibitor 9 (PI-9) messenger RNA (mRNA) and protein by Mycobacterium tuberculosis H37Rv lysate in alveolar macrophages (AMs) and blood monocytes (MNs). Induction of PI-9 mRNA and protein in AMs and MNs exposed to M. tuberculosis H37Rv lysate (1 μg/mL) was assessed. A, Induction of PI-9 mRNA in AMs and MNs after 4 hours of incubation with 1 μg/mL H37Rv lysate (solid bars) or medium alone (striped bars). B, Concentrations of PI-9 protein (as measured by enzyme-linked immunosorbent assay) in cell lysates of AMs and MNs after 24 hours of culture with H37Rv lysate or medium. Differences in the induction of MN PI-9 mRNA and PI-9 protein were both significant, as indicated.