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Lysates (1 mg) from HEK293T cells that had been transfected with the indicated plasmids were immunoprecipitated with an anti-V5 antibody. Immunoprecipitated complexes and cell lysates (50 µg) were analyzed by Western blotting using HA- and V5-specific antibodies. Asterisks: non-specific bands; #: immunoglobulin heavy chains; white arrowhead: expected migration of HA-G3BP1.
