Table 1. Inhibition of CHIKV replicon in BHK-CHIKV-NCT cells by hit and reference compounds.
| Compound | EGFP a IC50(µM) | Rluc aIC50(µM) | Cell viabilityb IC50 (µM) |
| NC compounds | |||
| Apigenin | 22.5 | 28.3 | >200 |
| Chrysin | 46.8 | 50.2 | >200 |
| Naringenin | 25.8 | 30.0 | 122.1 |
| Silybin | 71.1 | 59.8 | >200 |
| PC compounds | |||
| Prothipendyl | 135.0 | 93.3 | 185.6 |
| Reference compounds | |||
| Ribavirin | 8.8 | 25.4 | >200 |
| Mycophenolic acid | 1.5 | 4.1 | >200 |
| 6-Azauridine | 2.4 | 3.1 | >200 |
| 3′-NH2-3′-deoxyadenosine | 34.0 | 62.4 | 187.1 |
aIC50 values for suppression of CHIKV replicon were determined by exposing the replicon cell line to test compounds at various concentrations from 200 µM to 10 nM for 48 h.
bCell viability IC50 values were determined by ATP assay after 48 h exposure of BHK-CHIKV-NCT cells. All results represent the mean values from two individual experiments both run in triplicate (CV ranged from 4.9 to 13.7% in the CHIKV replicon assay). NC = natural compounds, PC = pharmaceutical compounds.