Figure 3.

Inactivation of mTORC1 and autophagy induction in Pah^enu2 mice. (A and B) The growth of 25-d-old Pah^enu2 mice was compared with that of 25-d-old wild-type mice (n = 6). Data represent means ± SD **p < 0.01, compared with the control group. (C) Inactivation of mTORC1 and induction of autophagy in Pah^enu2 mice. Liver and muscle homogenates from 25-d-old wild-type or Pah^enu2 mice fed a normal diet were analyzed by protein gel blotting to examine the phosphorylation of S6K and the conversion of LC3-I into LC3-II. (D) Band intensities of protein gel blot in (C) were quantified and ratios of p-S6K/S6K and LC3-II/LC3-I were presented. Values represent means ± SD (n = 3 experiments). *p < 0.05, compared with wild-type mice.