Table 3.

Validation of site-specific CpG differential placental methylation by virtue of maternal smoking

CpG Genomic Location	Gene Annotation	Gene	Biological Function	Delta Beta (array estimate)	Diff Score	Delta Beta (sequence validation)
5:139474190	PURA	purine-rich element binding protein A	DNA unwinding during replication	0.187	341.0777	0.108
5:70399433	GTF2H2	general transcription factor IIH	nucleotide-excision repair	0.196	341.0777	0.146
2:162909221	GCA	grancalcin, EF-hand calcium binding protein	cellular membrane fusion	0.130	341.0777	0.082
14:59001866	GPR135	G protein-coupled receptor 135	G-protein coupled receptor activity	0.1299	341.0777	0.126
19:42517519	HKR1	GLI-Kruppel zinc finger family member	regulation of transcription	−0.19	−338.389	−0.25