Table 1.

List of primers used in this study

For RT-PCR

NAA10-F: ATG AAC ATC CGC AAT GCG AGG

NAA10-R: CTA GGA GGC TGA GTC GGA GG

NAA11-F: CAG CAC ACT TTC TGA TTC TGA AG

NAA11-R: GTA ATG GCA GGT CTC AAA GTT C

GAPD-5: ACC ACA GTC CAT GCC ATC AC

GAPD-3: TCC ACC ACC CTG TTG CTG TA

For cloning of NAA11 promoter*

NAA11-pro-F: TAA GGC ACG CGT TGT TAT ATG GGT TTC TCC AGT TTG TG

NAA11-pro-R: TAA GGC AAG CTT CTG AGC GTT GCG GAT GTT C

For DNA sequencing

M13-R: GGA AAC AGC TAT GAC CAT

M13-20: GTA AAA CGA CGG CCA GTG

For MS-PCR

M-NAA10-1F: GTT TCG CGT ATT GGT TAA GC

M-NAA10-1R: GTA AAA ACG CAA TCA ACT ACC G

U-NAA10-1F: GTT TGT TTT GTG TAT TGG TTA AGT G

U-NAA10-1R: CAT AAA AAC ACA ATC AAC TAC CAC C

M-NAA11-F: TGC GTA CGT ATA CGT ATA ATG AAG C

M-NAA11-R: ATA AAA AAC GAA TAA CGA AAA AAC G

U-NAA11-F: GTG TAT GTA TAT GTA TAA TGA AGT GA

U-NAA11-R: AAA AAA CAA ATA ACA AAA AAA CAA A

For bisulfite sequencing

BSS-NAA11-F: ATT TAA ATT AAA AAG TTA AAA TTG AAA TAA

BSS-NAA11-R: AAA AAA CAC TAT TTA CCT CAA AAA TC

BSS-NAA11-NR: AAT CCA AAA AAC TAA CAC CAC C

All primers are listed in 5′ to 3′ orientation.

^*Underlined sequence represents a random hexameric sequence included to protect the downstream MluI (ACGCGT) and HindIII (AAGCTT) restriction sites.