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. Author manuscript; available in PMC: 2012 Dec 15.
Published in final edited form as: Cancer Res. 2011 Oct 28;71(24):7513–7524. doi: 10.1158/0008-5472.CAN-11-1783

Fig. 5. Consequential upregulation of Cyclin D3 in ErbB2-induced mammary tumors that lack expression of Cyclin D1.

Fig. 5

A. Western blot analysis of D-type cyclins and Cyclin E in ErbB2-indcued primary mammary tumors that originated in the presence or absence of endogenous Cyclin D1. B. Western blot on explanted MMTV-neu-induced mammary tumor cells that were infected with three different Cyclin D3 shRNA knockdown vectors. pLKO1, control vector; cancer cells from a Cyclin D1 knockout mouse (D1−/−) were used as a positive/negative control for cyclins D1 and D3. NIH3T3 cells with and without one of the shRNA vectors served as an additional control. Note that Cyclin D2, which migrates below the D1, is absent in ErbB2-expressing cancer cells.