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. Author manuscript; available in PMC: 2013 Feb 1.
Published in final edited form as: Comp Biochem Physiol A Mol Integr Physiol. 2011 Nov 16;161(2):265–270. doi: 10.1016/j.cbpa.2011.11.004

Table 2.

O2 affinity (P50, torr; mean ±s.e.m.) and cooperativity coefficients (n50) for purified hemolysates measured in 0.1 M Hepes buffer at pH 7.40, 37 °C, in the presence of the met-Hb reducing system (Hayashi et al., 1973). Measurements were conducted in the absence of DPG and KCl (stripped), and in the presence of DPG (DPG/Hb tetramer ratio = 2.0) or 0.1 M KCl, or both effectors. [Heme], 0.16 mM. N=3 replicate measurements for C57BL/6J, BALB/cJ, and MSM/Ms.

C57BL/6J BALB/cJ MSM/Ms CAROLI/EiJ
P50 (torr)
 stripped 7.63±0.09 8.44±0.09 8.76±0.04 8.10
 DPG 10.95±0.42 11.88±0.17 11.64±0.07 12.48
 KCl 17.15±0.49 16.27±0.30 17.74±0.06 17.46
 DPG+KCl 16.87±0.48 17.53±0.29 17.03±0.27 18.45

Δ log P50
 DPG-stripped 0.16 0.15 0.12 0.19
 KCl -stripped 0.35 0.29 0.31 0.33
 (DPG+KCl) - stripped 0.34 0.31 0.29 0.36
 (DPG+KCl) - KCl −0.01 0.03 −0.02 0.02
 (DPG+KCl) - DPG 0.19 0.17 0.17 0.17

n50
 stripped 2.24±0.03 2.46±0.05 2.40±0.02 2.28
 DPG 2.44±0.084 2.65±0.09 2.50±0.04 2.53
 KCl 2.36±0.166 2.68±0.12 2.67±0.02 2.57
 DPG + KCl 2.45±0.011 2.78±0.13 2.56±0.09 1.91