Figure 3.

Ustekinumab discovery, cell line generation and antibody production. Ustekinumab is a human monoclonal antibody (mAb) discovered through the generation of hybridoma cultures from immunized human immunoglobulin (hu-Ig) transgenic (Tg) mice. Hybridomas secreting human mAbs that neutralized interleukin-12 (IL-12) were identified. Ustekinumab variable (V) and constant (C) domains were cloned from hybridoma cells and the heavy (H) and light (L) chains of the V and C domains were transfected into new host cells by electroporation. Ustekinumab is produced using perfusion fermentation culture and purified from the supernatant generated from the fermentation process.