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. Author manuscript; available in PMC: 2013 Jan 15.
Published in final edited form as: Respir Physiol Neurobiol. 2011 Oct 25;180(1):88–96. doi: 10.1016/j.resp.2011.10.015

Fig. 4.

Fig. 4

Motor end-plates at type-identified rat and mouse DIAm fibers. Representative confocal photomicrographs of neuromuscular junctions (NMJ) and type-identified DIAm fibers. Motor end-plates were labeled with Alexa 488-conjugated α-bungarotoxin and fiber types were identified by immunoreactivity to MyHCSlow or MyHC2A isoforms (type I or IIa fibers). Unlabeled fibers express MyHC2X and/or MyHC2B isoforms (type IIx and/or IIb fibers; see Methods). Maximum intensity projections showing significant differences in NMJ size, NMJ morphology and fiber dimensions across fiber types in the rat DIAm (left) but more similar NMJ size and morphology across fibers types in the mouse DIAm (right). Bar, 20 µm.