Figure 4.

Asymmetric localization of core PCP proteins. (A) Fz (pink) and Stbm (blue) are transiently asymmetrically localized in wing cells with Fz localizing to the distal apical membrane and Stbm localizing proximally. (B) In the five-cell precluster of the eye imaginal disc, expression of Fz and Stbm are initially equivalent in the presumptive R3/R4 cells but as development continues, the ommatidium starts to rotate and Fz becomes localized to the R3 side of the R3/R4 membrane border while Stbm localizes to the R4 cell membrane. (C) In the SOP of the bristle Fz localizes to the posterior membrane and Stbm localizes to the anterior membrane before asymmetric division. Where tested, Dsh and Dgo colocalize with Fz, while Pk colocalizes with Stbm (for details see text).