Figure 4.

Effect of vesicle traffic inhibition on P2X3 receptor turnover and its temperature sensitivity. (A) Typical examples of P2X3 receptor recovery traces at 21°C (tau = 2.5 min) and 29°C (tau = 2.8 min) after application of Brefeldin A (10 μg/ml). (B) Effect of Brefeldin A on the time constant of P2X3-DsRed fluorescence recovery after photobleaching. For comparison, control levels in (B) are shown as dotted lines. The asterisk indicates a significant difference from control values at P < 0.05.