Fig. 4.

Rho kinase plays different roles on contralateral sides of the gut. (A-C) Stage 38 Xenopus embryos were exposed to cRO, rinsed, and kept in the dark (A) or irradiated on the left (B) or right (C) side of the prospective gut, as in Fig. 2. The arc of the midgut curvature is indicated by the dashed line. Inset (C) shows a reversed curvature caused by ectopic concavity (arrow). (D) Average curvature for each condition (n≥10) was quantified as 1/radius of the best-fit circle superimposed on the greater curvature of the midgut. *, P<0.05; **, P<0.01 (one-way ANOVA); error bars indicate s.d.