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Double immunofluorescence staining of E22.5-day-old EBs in the presence of VEGF-A and VEGF-C. Representative images from three different experiments sets for each marker genes are shown. Arrowheads indicate MECA-32 positive blood vessel-like structures in E22.5-day-old EBs grown under both VN (A) and VH (B) treatments. Prox1 positive LECs are in close proximity to the blood vessel-like structures. Images were taken at 40× magnification (scale bar 30 μm).
