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. 2011 Dec 20;2:103. doi: 10.3389/fphys.2011.00103

Figure 7.

Figure 7

Double immunofluorescent staining of E36.5-day-old hypoxic EBs grown on collagen-I coverslips and treated with VEGF-A/C. Representative images from three different experiments sets for each marker genes are shown. (A,B) Collagen-I promotes predominantly the organization of LYVE1 positive lymphatic vessel-like structures (marked by arrowheads) when stimulated under hypoxic and VEGF-A/C environments. There are some traces of MECA-32 positive BECs along with these lymphatic vessel-like structures. Unlike E22.5-day-old EBs, all four types of treatment at E36.5 failed to form organized blood vessel-like structures. Images were taken at 40× magnification (scale bar 30 μm).