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. 2011 Dec 20;2:103. doi: 10.3389/fphys.2011.00103

Figure 8.

Figure 8

E36.5-day-old EBs grown on laminin. EBs did not form vessel-like structures when stimulated under normoxic, hypoxic and VEGF-A/C environments. Representative data from three different experiments sets for each marker genes are shown. (A) Normoxic EBs grown on laminin did not show any distinguishable LEC phenotype, similar to those grown on collagen-I. (B) Hypoxic EBs did not induce any vessel-like structures within differentiating EBs. (C) N + VEGF-A/C EBs formed few ring-like structures (arrowheads) within the core of the EB and showed staining for LYVE1 in certain regions. (D) H + VEGF-A/C environments promoted LEC (LYVE1+) differentiation mostly on the periphery of differentiating EBs take. Images were en at 40× magnification (scale bar 30 μm).