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. 2011 Dec 20;2:103. doi: 10.3389/fphys.2011.00103

Figure 9.

Figure 9

Triple immunofluorescence staining (20× magnification) of whole-mount mesenteric preparations of 3-month adult mice visualizing lymphatic vessels using LEC markers (A) LYVE1 (B) podoplanin and blood vessels using BEC marker CD31. Representative images from three different experiments sets for each marker genes are shown. Muscle cells are identified with α-vascular actin. The adult mouse mesentery demonstrates a complex network of blood and lymphatic vessels of different sizes. Initial lymphatic vessels (LYVE1+/Podoplanin+) are devoid of muscle cells (α-vascular actin−) whereas collecting lymphatic vessels (LYVE1−/Podoplanin+) show a nice arrangement of muscle cells (α-vascular actin+). Images were taken at 20× magnification (scale bar 12 μm).