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. Author manuscript; available in PMC: 2013 May 1.
Published in final edited form as: Cancer Lett. 2011 Dec 3;318(1):26–33. doi: 10.1016/j.canlet.2011.11.032

Figure 1. DLK1 loss-of-function in NB cells increases the potential for neuronal differentiation in vivo.

Figure 1

Parafin-embedded sections of (a) BE(2)C xenografts (Ctrl, DLK1-DM or DLK1-ΔCyto) or (b) ER xenografts (Ctrl or DLK-FL) were incubated with an anti-neurofilament (NF) antibody as described in Materials and Methods. Bar = 100 µm. NF+ cells were counted from three random fields (mean ± sem, **p<0.001; ***p<0.0001 versus control). (c) Co-immunofluorescence of DLK1 (red) and neurofilament (green) was performed in frozen tumor sections as described in Materials and Methods (bar = 100 µm). DLK1+ cells (white triangles) do not express NF whereas NF+ cells (yellow arrowheads) do not express DLK1.