Figure 15.

SHP expression is negatively regulated by FUL and RPL. (A) Early in development the SHP genes are broadly expressed in the gynoecium. At stage 10, their expression extends throughout the valve margins, replum, septum, and developing ovules. Weak expression is also seen extending into the edges of the valves. (B) At stage 12, SHP expression is limited specifically to the valve margins. SHP also continues to be expressed in the developing ovules. (C) SHP continues to be expressed in the valve margins through stage 17. (D) Ectopic expression of FUL in 35S::FUL fruit is sufficient to block expression of SHP in the valve margins. (E) In rpl mutants, SHP expression is similar to wild type in early stages. (F) At stage 12 in rpl mutants, SHP continues to be expressed in the replum indicating that RPL is required to negatively regulate SHP expression in the replum. SHP is ectopically expressed in the replum region of rpl mutants through stage 17 (not shown). (G) In ful mutants, SHP is ectopically expressed throughout the valves indicating that FUL is required to negatively regulate SHP expression in the valves. (H) In rpl ful double mutants SHP expression completely surrounds the fruit.