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. 2006 Feb 22;4:e0075. doi: 10.1199/tab.0075

Figure 27.

Figure 27.

STYLISH specifies style development. (A) SEM of the apex of a wild-type gynoecium at stage 13. The stigma, style, valves and replum have been marked. (B) SEM of the apex of a sty1 gynoecium at stage 13 showing the presence of style cells on the top of the gynoecium (arrowhead) and reduced disorganized stigmatic papillae. (C) SEM of the apex of a sty1 sty2 double mutant at stage 13 showing that the style has formed short horn-like protrusions (arrowhead) and clustered stigmatic papillae. The amount of style and stigmatic tissue is reduced in comparison to wild type. (D) Vascular pattern of a cleared wild-type gynoecium at stage 13. Note that the medial vascular bundle bifurcates (arrowhead) immediately below the style where large xylem fans form. (E) The vascular pattern of sty1 sty2 double mutants showing basalized bifurcation of the medial vasculature (arrowheads) and reduced or absent xylem fans in the style. (F) SEM of a 35S::STY1 gynoecium at stage 13. Regions with ectopic style are bordered with a white line. (G) 35S::STY1 fruit dehiscing. The ectopic style cells block dehiscence (arrowhead and arrow). (H) Transmission electron micrograph (TEM) of a transverse section of wild-type valve epidermal cells showing smooth cell walls in the absence of wax crenulations. (I) TEM of wild-type style epidermal cells showing the wax crenulations characteristic of style cells. (J) TEM of 35S::STY1 valve epidermal cells in the region of ectopic style development showing that these cells form the wax crenulations characteristic of style cells. (K) STY1::GUS reporter line showing expression in the stigma and style of stage 12 gynoecium. (L) Inflorescence showing the expression of the STY2::GUS reporter in the style, stigma, and anthers of the flowers. (M) Representation of the STY1 expression pattern as seen through in situ hybridization. STY1 is expressed throughout the top of the gynoecium at stage 8. Expression is stronger in the medial regions than in the lateral regions. Scale bars in A-G represent 100 µm. Pictures H-J are taken at magnification 5200x. A-L: from Kuusk et al., 2002.