Figure 7.

Typical cytological features of RPW8-mediated powdery mildew resistance in ecotype Ms-0.

Whole-cell hydrogen peroxide (H₂O₂) accumulation in a G. orontii-attacked leaf epidermal cell of the Ms-0 ecotype (A), which is known to express RPW8-based powdery mildew resistance (Xiao et al., 2001). H₂O₂ accumulation is highlighted as a brownish 3,3-diaminobenzidine (DAB) precipitate (Thordal-Christensen et al., 1997) inside the challenged cell (arrowhead). Trailing necrosis of G. orontii-challenged leaf epidermal cells highlighted by Trypan-blue staining (B). Exemplary dead cells are highlighted by arrowheads. Scale bars: 100 μm