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. 2011 Nov 30;10:24. doi: 10.4103/1477-3163.90437

Figure 4.

Figure 4

Effects of D1 on BIRC5 and several members of the NF-κ B and Bcl-2 families. (a) RT-PCR and western blot assays show that Ela-mycPT1 cells transfected with D1 cDNA express increased D1 mRNA (D1-m) and protein (D1-p) levels, compared with the cells transfected with the pcDNA3.1-neo empty vector. HPRT1 or β-actin is used as a loading control. (b) RT-PCR detection of the mRNA levels of the RelA, RelB, Nfκb1, Nfκb2, IκBα , Bad, MCl-1, Bcl-xL, Bcl-xβ , Bcl-2, Bcl-2β , BIRC5 and Noxa in the D1-transfected Ela-mycPT1 cells and the vector-transfected counterparts, with HRPT1 as the loading control. (c) RT-PCR detection of the mRNA levels of the RelA, RelB, Nfκb1, Nfκ b2, Mcl-1, Bcl-2, Bcl-2β , Bad, Bcl-xL and Bcl-xβ in the D1 expressing D1.5 cells and the vector expressing Neo1 cells, with HPRT1 as the loading control. (d) RT-PCR or western blot detection of the c-myc mRNA or c-Myc protein level in the D1 cDNA or pcDNA3.1-neo vector transfected Ela-mycPT1 cells as well as in the D1.5 and Neo1 clones, with HPRT1 or β-actin as the corresponding loading control.