Figure 6.

Effects of CDK4 and CDK6 inhibitor NPCD on the regulation of the apoptosis regulating genes by D1. (a) RT-PCR detection of the mRNA levels of the RelA, RelB, Nfκb1, Nfκb2, Bad, Mcl-1, Bcl-2, Noxa, BIRC5 and c-myc in the D1 cDNA or vector transfected Ela-mycPT1 cells treated with both CDDP and NPCD for 48 hours. (b) RT-PCR detection of the mRNA levels of the above mentioned genes in D1.5 and Neo1 clones treated with both CDDP and NPCD for 48 hours. (c and d) RT-PCR detection of the mRNA levels of the RelA, RelB, Nfκb1, Nfκb2, Bad, Mcl-1, Bcl-2, Noxa and BIRC5 in the D1 cDNA transfected Ela-mycPT1 (c) and D1.5 cells (d) treated with NPCD alone. HPRT1 is used as the loading control for all the above RT-PCR assays