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. 2011 Nov 30;10:24. doi: 10.4103/1477-3163.90437

Figure 7.

Figure 7

Effects of c-Myc protein on the expression of apoptosis regulating genes. (a) RT-PCR and western blot assays show a higher c-myc mRNA (c-myc) and protein (c-Myc) level in the c-myc cDNA transfected Ela-mycPT1 cells, compared with the pcDNA3.1-hygro vector transfected counterparts. HPRT1 or β-actin is used as a loading control. (b) RT-PCR detection of the mRNA levels of the RelA, RelB, Nfκb1, Nfκb2, Mcl-1, Bcl-xβ, Bcl-xL, Bcl-2, and Bcl-2β in c-myc cDNA or the empty pcDNA3.1-hygro vector transfected Ela-mycPT1 cells, with HPRT1 as the loading control. (c) the c-myc mRNA (c-myc) or protein (c-Myc) level in the H1 and M8 clones of the Ela-mycPT4 cells, with HPRT1 or β-actin as the corresponding loading control. (d) RT-PCR detection of the mRNA levels of the Nfκ b1, Nfκ b2, RelA, RelB, Bcl-2β , Bcl-2, Bcl-xβ, Bcl-xL, Mcl-1, Bad, Noxa and BIRC5 genes in the H1 and M8 clones. (e) The D1 mRNA (D1-m) and protein (D1-p) level is lower in the c-myc cDNA transfected Ela-mycPT1 cells as well as in M4 and M8 c-myc expressing cell clones, in relative to the corresponding vector (pcDNA3.1-hygro and H1) counterparts. HPRT1 or β-actin is used as a loading control.