Figure 2.

Morphological change of plastid nucleoids in the first true leaves of Arabidopsis.

(A) Plant material used for preparing thin sections. An eight-day-old Arabidopsis seedling (left; a top view is shown) was selected and the two cotyledons were removed (as indicated by black lines). One of the first true leaves of this same plant (right; a side view is shown) was used for the detection of plastid nucleoids.

(B) A thin cross-section of the selected true leaf petiole and lamina, stained by Toluidine blue. The positions indicated by the arrows (1 to 3) were further examined by SYBR-green I staining (as shown in C).

(C) Examination of thin sections by SYRB green I. Signals corresponding to nuclear and plastid DNAs are shown by red and yellow arrows, respectively. Close-up views of the plastids in the respective areas (1 to 3) are shown below each panel.