Table 2.
TRAF-2 gene and protein expression in NDC and Dc SH-SY5Y cells after TNF-α treatment
TNF-α | TRAF-2 | |||
---|---|---|---|---|
NDC | DC | |||
Gene | protein | gene | protein | |
(fold change) | Mean % of total (SEM) | (fold change) | Mean % of total (SEM) | |
Control | ‡1 | +12.39 | 1 | 13.48 |
(0.75-1.34) | (0.09) | (0.82-1.22) | (0.06) | |
1 ng, | 1.03 | 13.39 | 1.11 | 12.44 |
15 min | (0.66-1.61) | (0.13) | (0.79-1.55) | (0.14) |
10 ng, | 1.01 | 14.31 | 1.09 | 13.33 |
15 min | (0.72-1.42) | (0.08) | (0.75-1.57) | (0.14) |
50 ng, | 1.08 | 14.57 | 0.88 | 13.70 |
15 min | (0.74-1.57) | (0.15) | (0.78-0.98) | (0.08) |
1 ng, 3h | 1.15 | 13.51 | 1.33 | 13.48 |
(0.71-1.87) | (0.09) | (0.74-2.39) | (0.09) | |
10 ng, 3h | 1.49 | 14.44 | 1.71* | 15.86 |
(1.06-2.08) | (0.10) | (1.08-2.72) | (0.15) | |
50 ng, 3h | 1.59 | 16.30 | 1.91** | 17.72 |
(1.03-2.46) | (0.04) | (1.22-2.98) | (0.11) |
Numbers are the geometric means (with 95% confidence intervals) of the fold change in gene expression and the + mean % of total protein as measured by Image J software with standard error of the mean (SEM) in each set of experiments.
p=0.032 (Mann-Whintney)
p=0.008 (Mann-Whitney)