Table 3.
ECE-2 gene and protein expression in NDC and DC SHSY5Y cells treated with TNF-α
| ECE-2 | ||||
|---|---|---|---|---|
| TNF-α | NDC | DC | ||
| gene | protein | gene | protein | |
| (fold change) | mean (ng/ml) (SEM) | (fold change) | mean (ng/ml) (SEM) | |
| Control | ‡1 (0.74-1.34) | 166.85 (12.05) | 1 (0.47-2.12) | 54.25 (7.19) |
| 1 ng, 15 min | 0.93 (0.63-1.38) | 116.70* (9.21) | 1.00 (0.41-2.48) | 73.73 (9.9) |
| 10 ng, 15 min | 0.95 (0.72-1.23) | 104.91** (8.9) | 1.04 (0.43-2.51) | 98.21 (11.41) |
| 50 ng, 15 min | 0.94 (0.68-1.29) | 80.16*** (8.9) | 1.13 (0.6-2.1) | 86.54 (7.55) |
| 1 ng, 3h | 0.75 (0.55-1.02) | 175.2 (10.02) | 1.06 (0.52-2.17) | 80.81 (8.0) |
| 10 ng, 3h | 0.88 (0.62-1.26) | 181.2 (13.79) | 1.16 (0.55-2.44) | 78.31 (6.12) |
| 50 ng, 3h | 0.94 (0.63-1.4) | 201.0 (10.9) | 1.47 (0.73-2.95) | 66.75 (12.11) |
‡
Numbers showing gene expression are geometric means (with 95% confidence intervals) and those showing protein concentration are arithmetic means and the standard error of the mean (SEM).
*
p = 0.016
**
p = 0.002
***
p = 0.002 (Mann-Whitney test)