EI-MS/MS spectra of very long chain anteiso FAME. (A) Mass spectrum of n-25:0, (B) iso-25:0, and (C) anteiso-25:0. The observed fragments are analogous to those observed for shorter chain BCFAME. (D, E, F) Mass spectra of anteiso-27:0, anteiso-29:0, and anteiso-31:0 show similar fragments to shorter chain anteiso BCFAME, although signal-to-noise is lower for these rarer FAME. The m/z 115 ion is observed for most anteiso VLC-BCFAME.