Fig. 6.

Severe liver disease in hepatocyte-specific Pggt1b knockout mice (Pggt1b^Δ/Δ). (A, B) Hematoxylin and eosin–stained liver sections from 4-month-old Pggt1b^+/+ and Pggt1b^Δ/Δ mice, showing disordered hepatic lobules with dilated sinusoids and multiple blood-filled cavities. (C, D) PAS stain revealing increased glycogen (magenta) in the liver of a Pggt1b^Δ/Δ mouse. Images in panels A–D were recorded with a 20× objective. Scale bar, 50 µm. (E, F) In vivo detection of active caspases 3 and 7 (green) in the livers of 4-month-old Pggt1b^+/+ and Pggt1b^Δ/Δ mice (n = 3 mice/group were examined). DNA was visualized with DAPI (blue). (G, H) Abnormal organization of actin fibrils in the liver of Pggt1b^Δ/Δ mice, as judged by phalloidin staining (green). DNA was visualized with DAPI (blue). Scale bar for images in panels E–H, 10 µm.