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. 2011 Oct 13;286(51):44266–44276. doi: 10.1074/jbc.M111.271593

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 2. — Pulldown assays further confirm that the specificity of the DISC1/NDEL1 interaction is mapped to a key helical region on the C terminus of DISC1. a, to map the NDEL1 binding region on recombinant DISC1, two DISC1 constructs (MBP-DISC1-(680–802) and MBP-DISC1-(680–832) were made based on the previous yeast two-hybrid studies (24). These expressed DISC1 proteins were subjected to pulldown assay using NDEL1-CTF (shown in an arrow). MBP-DISC1-CTF-(680–802), which lacks the crucial helical region (802–832), could not pull down NDEL1. b, proper folding of full-length DISC1 was confirmed using MBP-DISC1 as a bait to pull down full-length NDEL1. The interaction was being competed using C-terminal region of DISC1.