FIGURE 6.

Both in inner membrane vesicles as well as in intact cells, the transmembrane helix of TatA is in close proximity to TatC. A and B, the indicated vesicles were irradiated with UV light to initiate cross-linking to the Bpa variants of TatA. Homo-oligomeric TatA adducts that were revealed by immunoblots using anti-TatA antibodies (α TatA) are marked. C, as in A and B except that the blot is decorated with anti-TatB antibodies. TatA adducts recognized by anti-TatB antibodies are indicated. D, as in C except that TatA adducts recognized by anti-TatC antibodies are highlighted. E, as in D except that whole cells had been irradiated with UV light.