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. 2011 Nov 8;286(51):44277–44284. doi: 10.1074/jbc.M111.273979

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 1. — Wzz2 proteins produce different very long O antigen chain lengths. A, Western blot of LPS isolated from the wild-type P. aeruginosa serotype O11 strains and the DΔM complemented with the two wild-type Wzz2 proteins. The top arrow indicates the longer “very long” chain length produced by PA103, whereas the bottom arrow marks the shorter very long chain length produced by 9882-80. Lanes are as follows: lane 1, PA103; lane 2, 9882-80; lane 3, DΔM+pHERD30T; lane 4, DΔM+pPA103 wzz2; lane 5, DΔM+p9882-80 wzz2. B, TCOFFEE alignment between PA103 Wzz2 and 9882-80 Wzz2. Transmembrane domains are indicated by the black bars, and the coiled-coil regions (predicted using the COILS program) are outlined in boxes. Sites chosen for site-directed mutagenesis are marked with arrows.