TABLE 1.

SHMT1 overexpressing mice are viable

Shmt1^tg mice were crossed with Cre-expressing mice to activate expression of the Shmt1 transgene, and their progeny were genotyped. The expected genotype distribution was calculated based on a Mendelian distribution. Differences between observed and expected genotype distributions were analyzed by chi-squared analysis. p values ≤ 0.05 were considered significantly different. Shmt1^wt+, Cre⁺ Shmt1 wild type mice; Shmt1^tg+, Cre⁺ Shmt1^tg mice. NS, not significant.

Genotype	Observed genotype distribution			Expected genotype distribution
	Male	Female	Total	Male	Female	Total
Shmt1wt+	55	42	97	56.5	56.5	113
Shmt1tg+	70	59	129	56.5	56.6	113
Total	125	101	226	113	113	226
Number of litters observed				39
Mean litter size (mean ± S.E.)				5.8 ± 0.4
p value, observed versus expected genotype distribution				NS
p value, observed versus expected sex distribution				NS