TABLE 2.

Plasma and liver folate, liver S-adenosyl-methionine, S-adenosyl-homocysteine, S-adenosyl-methionine:S-adenosyl-homocysteine ratio, and uracil content in SHMT1-overexpressing mice at 32 weeks post-weaning

Differences between genotypes and diets were analyzed by Student's t test. nuclear DNA genotype × diet effects were analyzed by two-way ANOVA using Tukey's Honestly Significant Difference post-hoc analysis. The data represent the means ± S.E. values. p values ≤ 0.05 were considered significantly different. n = 4–7/group. Folate/choline-deficient Shmt1^tg+ mice are significantly different than control and folate/choline-deficient Shmt1^wt+ and control Shmt1^tg+ mice, p < 0.05, as analyzed by two-way ANOVA and Tukey's Honestly Significant Difference post-hoc test Shmt1^wt+, Cre⁺ Shmt1 wild type mice; Shmt1^tg+, Cre⁺ Shmt1^tg mice. NS, not significant.

Diet	Shmt1 genotype	Plasma folate	Liver folate	AdoMet	AdoHcy	AdoMet:AdoHcy	Liver uracil
		ng/ml	fmol/μg of protein	pmol/μg of protein	pmol/μg of protein		pg of uracil/μg of DNA
AIN-93G	Shmt1wt+	36.3 ± 7.7	43.1 ± 2.3	0.7 ± 0.2	0.4 ± 0.1	2.1 ± 0.4	0.1 ± 0.0
	Shmt1tg+	46.8 ± 5.8	51.3 ± 3.4	0.3 ± 0.2	0.2 ± 0.2	1.2 ± 0.0	0.2 ± 0.0
AIN-93G minus folate & choline	Shmt1wt+	7.4 ± 0.2	36.1 ± 2.6	0.7 ± 0.1	0.6 ± 0.1	1.2 ± 0.2	0.3 ± 0.0
	Shmt1tg+	5.5 ± 1.5	34.0 ± 5.8	0.4 ± 0.0	0.6 ± 0.1	0.9 ± 0.3	2.3 ± 0.7
p value, diet effect		<0.0001	<0.0001	NS	0.006	0.04	0.01
p value, genotype effect		NS	NS	0.02	NS	0.04	0.02
p value, Diet × genotype effect		NS	NS	NS	NS	NS	0.031