TABLE 3.
Plasma metabolic profile of SHMT1 overexpressing mice at 5 weeks post-weaning
Differences between sexes, diets, and genotypes were analyzed by Student's t test. Genotype × diet effects were analyzed by two-way ANOVA using Tukey's Honestly Significant Difference post-hoc analysis. The data are presented as the means ± S.E. values. p values ≤ 0.05 were considered significantly different. n = 3 males and n = 3 females/diet/genotype group. Shmt1wt+, Cre+ Shmt1 wild type mice; Shmt1tg+, Cre+ Shmt1tg mice. NS, not significant.
| Genotype |
Shmt1wt+ |
Shmt1tg+ |
p value of model effect |
||||||
|---|---|---|---|---|---|---|---|---|---|
| Metabolite | Sex | C | FCD | C | FCD | Sex | Diet | Genotype | Diet × genotype |
| Homocysteine (μm) | Both | 5.6 ± 0.7 | 7.0 ± 0.7 | 7.8 ± 1.3 | 9.9 ± 1.0 | 0.009 | 0.007 | 0.06 | NS |
| Male | 4.8 ± 0.7 | 5.5 ± 0.2 | 6.6 ± 2.3 | 8.4 ± 0.6 | 0.10 | NS | NS | ||
| Female | 6.4 ± 1.0 | 8.4 ± 0.3 | 9.0 ± 1.1 | 11.4 ± 1.6 | 0.03 | 0.08 | NS | ||
| Cystathionine (nm) | Both | 1697 ± 237 | 1687 ± 334 | 1552 ± 189 | 1555 ± 70 | 0.002 | NS | NS | NS |
| Male | 1979 ± 399 | 2235 ± 483 | 1931 ± 181 | 1655 ± 83 | NS | NS | NS | ||
| Female | 1414 ± 202 | 1140 ± 159 | 1173 ± 54 | 1455 ± 89 | NS | NS | 0.08 | ||
| Cysteine (μm) | Both | 171 ± 29 | 200 ± 20 | 214 ± 19 | 219 ± 15 | <0.0001 | 0.006 | 0.10 | NS |
| Male | 109 ± 21 | 158 ± 8 | 178 ± 15 | 192 ± 14 | 0.01 | 0.07 | NS | ||
| Female | 232 ± 4 | 242 ± 12 | 251 ± 18 | 246 ± 12 | NS | NS | NS | ||
| Methionine (μm) | Both | 39.6 ± 5.0 | 29.3 ± 3.9 | 40.9 ± 8.8 | 31.4 ± 1.3 | 0.03 | NS | 0.07 | NS |
| Male | 49.9 ± 3.7 | 34.6 ± 5.8 | 48.1 ± 18.2 | 32.4 ± 1.6 | NS | NS | NS | ||
| Female | 29.3 ± 2.2 | 24.1 ± 3.7 | 33.7 ± 1.1 | 30.4 ± 2.2 | 0.07 | NS | NS | ||
| α-Aminobutyric Acid (μm) | Both | 6.8 ± 1.7 | 5.0 ± 0.5 | 4.5 ± 0.6 | 4.2 ± 0.3 | NS | NS | NS | NS |
| Male | 5.8 ± 1.4 | 4.6 ± 0.4 | 5.0 ± 1.2 | 4.4 ± 0.5 | NS | NS | NS | ||
| Female | 7.8 ± 3.3 | 5.4 ± 1.0 | 4.0 ± 0.3 | 4.0 ± 0.3 | NS | NS | NS | ||
| Glycine (μm) | Both | 312 ± 42 | 286 ± 22 | 270 ± 17 | 289 ± 20 | 0.0006 | NS | NS | NS |
| Male | 383 ± 59 | 322 ± 32 | 291 ± 23 | 327 ± 6 | NS | NS | NS | ||
| Female | 240 ± 12 | 249 ± 9 | 249 ± 22 | 250 ± 20 | NS | NS | NS | ||
| Serine (μm) | Both | 161 ± 21 | 136 ± 11 | 146 ± 18 | 134 ± 9 | <0.0001 | NS | 0.07 | NS |
| Male | 203 ± 17 | 158 ± 8 | 174 ± 25 | 151 ± 6 | NS | 0.07 | NS | ||
| Female | 119 ± 10 | 113 ± 8 | 119 ± 16 | 117 ± 9 | NS | NS | NS | ||
| Dimethylglycine (μm) | Both | 7.8 ± 1.7 | 7.1 ± 0.7 | 6.0 ± 0.7 | 6.2 ± 0.8 | 0.0004 | 0.10 | NS | NS |
| Male | 5.0 ± 0.1 | 5.6 ± 0.4 | 5.0 ± 0.7 | 4.6 ± 0.2 | NS | NS | NS | ||
| Female | 10.5 ± 2.6 | 8.6 ± 0.6 | 7.0 ± 1.1 | 7.8 ± 0.5 | NS | NS | NS | ||
| Methylglycine (μm) | Both | 2.8 ± 0.5 | 2.1 ± 0.2 | 1.4 ± 0.1 | 2.0 ± 0.3 | NS | 0.04 | NS | 0.06 |
| Male | 2.3 ± 0.5 | 2.3 ± 0.3 | 1.4 ± 0.1 | 1.6 ± 0.1 | 0.02 | NS | NS | ||
| Female | 3.3 ± 1.0 | 1.8 ± 0.2 | 1.3 ± 0.1 | 2.4 ± 0.7 | NS | NS | 0.07 | ||