FIGURE 4.

EphrinA1/EphA2 signal inactivates RhoA that is required for maintaining the flat shape of MDCK cells. A, immunoblot analyses with the antibodies indicated at the left using the cell lysates of the MDCK cells treated with siRNAs indicated at the top. B, the confocal images of the cells transfected with siRNAs indicated at the top were obtained as described in the legend of Fig. 2B. C, area was calculated as described in the legend of Fig. 1D. D and E, pulldown analyses with the antibodies indicated at the left using the lysates of the MDCK cells stimulated with ephrinA1-Fc (D) or those cultured at different density (E).