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. 2011 Nov 2;286(51):43925–43932. doi: 10.1074/jbc.M111.264192

FIGURE 1.

FIGURE 1.

Generation of Col2-RB1CC1 transgenic mice. A, the Neo gene was flanked by two loxP sequences in CAG-floxed-Neo-RB1CC1 transgenic mice. The transgenic mice were crossed with Col2-Cre transgenic mice, expressing Cre recombinase under the promoter for collagen2 α1 (Col2a1), and Cre-mediated deletion of the Neo gene led to production of FLAG-tagged human RB1CC1 under the CAG promoter in the chondrocytes of Col2-RB1CC1 (RB1CC1/Cre) mice. B, genomic DNA was extracted from mouse tail and analyzed by PCR, as indicated, to distinguish different types of alleles resulting from Cre-mediated recombination. The top arrow indicates 2.2-kb amplified products of the RB1CC1 transgenic allele. The Neo-deleted RB1CC1 allele (arrowhead; 0.8 kbp) is also amplified from the RB1CC1/Cre mouse tail, which contains the chondrocytes. The bottom arrowhead (199 bp) and arrow (324 bp) indicate the amplified products of Col2-Cre transgene and Il2 gene on wild-type mouse chromosome 3 (GenBankTM accession number NT_162143.3; nucleotides 21,361,049–21,361,372).