FIGURE 6.

Human RB1CC1 promoter SNP modified the self-activation. A, luciferase reporter vectors containing each kind of SNP (T-547C, C-468T, C-377T, T-296C, or G-226A) on the human RB1CC1 promoter were constructed and transduced into ATDC5 chondrocytes. B, luciferase reporter assays demonstrated that T-547C significantly decreased the activity (mean ± S.E. (error bars) = 63.5 ± 5.7%) and that C-468T increased it (141.6 ± 7.1%), compared with the activity of common variant (wt). The means ± S.E. were from more than quadruplicate experiments. One-way factorial analysis of variance and multiple comparison tests accompanied by Scheffe's significance were used to evaluate the relationships between each SNP and the promoter activity (*, p < 0.05, statistically significant).