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. 2011 Oct 21;286(51):43717–43725. doi: 10.1074/jbc.M111.292755

FIGURE 2.

FIGURE 2.

Association of DHPR with JP1-B in skeletal muscle and in HEK293-T transfected cells. A, Western blot (IB) with an anti-α1s-DHPR antibody of solubilized rabbit skeletal muscle microsomes (M) and of proteins pulled down with GST-JP1-A, GST-JP1-B, GST-JP1-C, and GST alone, as negative control. DHPR, anti-α1s-DHPR antibody. B, Western blot with an anti-α1s-DHPR antibody of lysates of HEK293-T cells transfected with pEGFP-α1s-DHPR, pEGFP-β1a-DHPR, and pCDNA-JP1 (TL) and of proteins pulled down with GST-JP1-A, GST-JP1-B, GST-JP1-C, and GST alone, as negative control. C, Western blot with an anti-α1s-DHPR antibody of solubilized rabbit skeletal muscle microsomes and proteins pulled down with GST-JP2-A, GST-JP2-B, GST-JP2-C, and GST alone, as negative control.