FIGURE 3.

Immunofluorescence analysis of expression inhibition of JP1 and JP2 in C2C12 cells. C2C12 cells were transfected with pSuperLuc-GFP as negative control (A–C, G–I, M–O, and S–U) or with pSuperJPAi-GFP to silence JP1 and JP2 expression (D-F, J–L, P–R, and V–X). C2C12 cells transfected with pSuperLuc-GFP were immunostained with antibodies against JP1 (A–C), JP2 (G–I), RyR (M–O), and α1s DHPR (S–U). C2C12 cells were transfected with pSuperJPAi-GFP were immunostained with antibodies against JP1 (D–F), JP2 (J–L), RyR (P–R), and α1s-DHPR (V–X).