. 2011 Sep 30;286(51):44104–44115. doi: 10.1074/jbc.M111.234559

TABLE 2.

MDM2/MDMX ubiquitination of RFP-p53 and RFP-p53-Ub results in different ubiquitination pattern

MS analysis of the ubiquitination sites on RFP-p53 (left side) and RFP-p53-Ub (right side) upon ubiquitination in the presence and absence (−) of MDM2/MDMX is shown. ND, no modification detected.

E2	RFP-p53		RFP-p53-Ub
E2	MDM2	MDM2 + MDMX	−	MDM2
E2D2	Lys-101^a, Lys-120^a (p53)	Lys-319^a, Lys-320^a (p53); Lys-11^a, Lys-48^a (Ub)	Lys-164^b,c (RFP); Lys-6^b,c, Lys-11^b, Lys-48, Lys-27^b, Lys-29^b (Ub)	Lys-80^b,c (RFP); Lys-351^b,c (p53); Lys-6^b,c, Lys-11^b, Lys-48^b, Lys-63^b (Ub)
E2D3	Lys-11^b, Lys-48^b (61) (Ub)	Lys-80^b (RFP); Lys-6^b, Lys-11^b, Lys-48^b (Ub)		Lys-174^a (RFP); Lys-11^a, Lys-48^a (Ub)
E2N-E2V1	Lys-63^b (63)	Lys-80^b,c (RFP); Lys-142^b,c (MDM2); Lys-11^b,c, Lys-63^b (Ub)	Lys-319^b, Lys-320^b (p53); Lys-63^a (Ub)	Lys-63^a (Ub)

^a Ion trap data.

^b Orbitrap data.

^c Non-tryptic peptide.