FIGURE 3.

Uptake and killing activity of Hst 5 is reduced in cells cultured with spermidine and spermine. A, wild-type CAF4-2 cells were grown either in growth medium only (●) or in growth medium supplemented with putrescine, spermine, or spermidine (1 mm (□) and 2 mm (Δ)). Addition of putrescine to growth medium did not affect the sensitivity of C. albicans cells to Hst 5 mediated killing (p > 0.05). The presence of either spermine or spermidine in the growth medium significantly increased the resistance of C. albicans to Hst 5 toxicity, with the highest inhibition of killing in cells grown in spermidine. B, intracellular translocation of FITC-Hst 5 (30 μm) was reduced in cells grown in medium with added spermidine (Spd) (2 mm) (right panel) as compared with control cells grown in medium without the addition of spermidine (middle panel). Transcript levels of DUR3 were reduced in CAF4-2 cells grown in the presence of 2 mm spermidine, whereas there was no difference in the expression level of DUR31.