FIGURE 1.

Schematic illustration of PrP constructs, and analysis of PrP expression in transgenic mice. A, schematic of wild-type (WT), C1 (Δ23–111), and PrP(Δ23–134). Structural domains of PrP are indicated by the colored blocks: SS, signal sequence (blue); PBD, polybasic domain (yellow); OR, octapeptide repeats (orange); CC, charged cluster (red); HD, hydrophobic domain (green); GPI, GPI attachment signal (purple). The dotted lines indicate deleted regions. B, Western blot analysis of protein expression. Brain samples from mice of the indicated genotypes were normalized for total protein, treated with or without PNGase F to remove N-linked oligosaccharides (+ and − lanes, respectively), and subjected to Western blotting with anti-PrP antibody 6H4. Filled and open arrowheads to the left of lane 2 indicate the positions of cleavage products C1 and C2, respectively. The upper band in lane 8 (asterisk) represents residual, mono-glycosylated C1 that was not completely shifted by treatment with PNGase. Molecular size markers are given in kDa.