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. 2011 Oct 27;286(51):43690–43700. doi: 10.1074/jbc.M111.290437

FIGURE 6.

FIGURE 6.

Direct interaction of microRNA 15a and 16–1 with the PCMT1 3′ UTR seed sequence monitored by luciferase reporter activity assay. A construct of the PCMT1 3′ UTR carrying the putative seed sequence for miRNA 15a/16–1 interaction was cloned downstream of a luciferase reporter gene in pGL3TK vector. HepG2 were co-transfected with this recombinant plasmid and with either pre-miR 15a or 16–1. Levels of luciferase activity were normalized against the activities of a Renilla internal standard expressed by a co-transfectant pRLCMV vector in each sample. pGLpcmt/miR15, pGL3TK vector carrying the PCMT1 seed sequence, co-transfected with pre-miR15. pGLpcmt/miR16, pGL3TK vector carrying the PCMT1 seed sequence, co-transfected with pre-miR16. pGLCT/miR15, pGL3TK empty vector, co-transfected with pre-miR15 (control). pGLCT/miR16, pGL3TK empty vector, co-transfected with pre-miR16 (control). pGLpcmt/miRNARD, pGL3TK vector carrying the PCMT1 seed sequence, co-transfected with scrambled pre-miRNA (control).