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. 2011 Oct 27;286(51):43690–43700. doi: 10.1074/jbc.M111.290437

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 8. — Methyltransferase activity in HepG2 upon transfection with siRNAs and shRNAs against PCMT1. Cells were transfected with various siRNAs and shRNAs against PCMT1 and then treated with cisplatinum under condition A. Methyltransferase-specific activity was measured in cell extracts. A, C siRNA rd, negative control transfected with scrambled siRNA; siRNA pcmt3, cell sample transfected with siRNA against PCMT1 3′ UTR; siRNA pcmt5, cell sample transfected with siRNA against PCMT1 5′ UTR. *, p < 0.05. B, C shRNA RD, negative control transfected with scrambled shRNA; shRNAvs5′UTRpcmt(a), shRNA directed against the 5′ UTR of PCMT1 transcript; shRNAvs5′UTRpcmt(b), shRNA directed against a different 5′ UTR of PCMT1 transcript; shRNAvsORFpcmt(a), shRNA directed against the PCMT1 transcript ORF; shRNAvsORFpcmt(b), shRNA directed against a different sequence in the PCMT1 ORF. *, p < 0.05.