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HSS−7 associates with the nuclear matrix. A, nuclear matrices were isolated from J774 cells and digested with EcoRI, BamHI, HindIII, PstI, and NspI. The matrix fraction was then separated from the non-matrix-associated fraction by centrifugation. Purified matrix DNA and genomic DNA were amplified by PCR utilizing primers specific for HSS−7 and HSS−9 region 1. B, densitometric analysis of the bands is shown.
