FIGURE 4.

HSS−7 associates with acetylated histones, HMGA1a, and Top2 in vivo. ChIP assays were performed using antibodies prepared against peptides comprising the N-terminal tails of histone H3 acetylated at Lys-9 and Lys-14 (anti-acetylated histone H3), histone H4 acetylated at Lys-8 (anti-acetylated H4), or histones H3 and H4 in an unmodified state (anti-unacetylated histones H3 and H4, respectively). A, ChIP assay with unstimulated and Mycobacterium tuberculosis (MTb)-stimulated J774 cells using the anti-acetylated histone H3 (AcH3) or H4 (AcH4) antibody. B, ChIP assay with unstimulated J774 cells and the anti-HMGA1a or anti-Top2 antibody. Immunoprecipitation with the anti-unacetylated histone H3 (H3) or H4 (H4) antibody (A) and an isotype control antibody (A and B) served as negative controls. DNA was amplified with primers flanking HSS−7 (underlined in Fig. 6E).