FIGURE 3.

Bro1 localization and Rim101 cleavage are unaffected by NHX1 deletion. a, WT or the nhx1Δ, vps4Δ, or nhx1Δvps4Δ mutant were grown in medium adjusted to the indicated pH and analyzed for V5 epitope-tagged Rim101 by Western blotting. Note the increase in the proteolytically processed (p) band relative to the unprocessed (u) band at more alkaline pH. Alkaline pH-induced or constitutive (in vps4Δ) Rim101 processing was not affected by nhx1Δ. IB, immunoblot. b, Bro1-GFP localization in nhx1Δ is similar to that in WT. Endosomal accumulation of Bro1-GFP occurred in both vps4Δ and nhx1Δvps4Δ mutants.